Mutagenesis dengan Transposon pada Xanthomonas oryzae pv. oryzae Penyebab Hawar Daun Padi

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight (BLB) disease of rice that can reduce rice productivity. The use of biocontrol agents is an effective solution to solve this problem. The aim of this study was to obtain mutants of Xanthomonas oryzae pv oryzae through transposon mutagenesis. Transposon mini-Tn5Km1 carried by E.coli S-17-1 (λ pir) was introduced into Xanthomonas oryzae pv oryzae STG21 by conjugation diparental mating. Frequency of tranconjugation obtained from this study was about 2.7×10-6, 1.1×10-6, dan 2.4×10-6 at the ratios of 1:1, 1:10, and 10:1 respectively. Five mutants (M93, M99, M107, M112, and M120) out of 300 mutants tested did not cause a hypersensitive reaction on tobacco plants. However, on rice IR64 plants showed that those mutants could reduce the symptoms of BLB with presentage of 96%, 94%, 94%, 91% and 92% respectively.