Gambaran Histopatologi Organ Limpa pada Kalong Asal Gorontalo

Abstract

Acerodon celebensis and Pteropus hypomelanus are species of Chiroptera that lived at Gorontalo. Those species are known as fruit bats that has potential as reservoir host for several zoonosis cases. The aim of this study was to determine the health status of Gorontalo’s fruit bats through the overview histopathology of spleen. Nine samples were used in this research that consist of seven spleens of Acerodon celebensis and two spleens of Pteropus hypomelanus that had been fixed in a solution of Buffer Neutral Formalin 10%. Observations were made by looking at the state of the red pulp and white pulp by using three different staining is Hematoxylin-Eosin, Congo Red, and Periodic Acid Schiff. Nine samples were observed by using Hematoxylin-Eosin showed the presence of inflammation and excessive accumulation of hemosiderin pigment. The result of Congo Red staining showed two positive samples with the presence accumulation of amyloid and Periodic Acid Schiff staining showed six positive samples with the presence accumulation of carbohydrates. Three samples of six positive samples showed microorganisms were suspected belongs to systemic mycosis. The conclusions of this research showed chronic inflammation in fruit bats spleen and were suspected due to systemic infection of Blastomyces dermatitidis.