Selulosa Benzoat Berbasis Selulosa Mikrobial dan Aplikasinya sebagai Fase Diam Kromatografi Cair Kinerja Tinggi (KCKT)

Abstract

Nata de coco is one of the alternative sources for the provision of microbial cellulose. This research aims to modify microcrystalline cellulose from microbial cellulose by esterification technique and subsequent esterification products are use as stationary phase in high performance liquid chromatography (HPLC). Microcrystalline cellulose was prepared by hydrolysis of dry nata de coco with a solution of HCl 4% for 4 hours. Esterification techniques performed by reacting benzoyl chloride and microcrystalline cellulose during 24 hours. Esterification products (cellulose benzoate) have a degree of benzoylation value is 2.84. Furthermore, cellulose benzoate used as the stationary phase in HPLC using a fluorescence detector for rhodamine b and erythrosine separation. The result showed that the average value of resolution was 1.150. Stationary phase obtained in this study was still not efficient for erythrosine and rhodamine b separation. It was seen from the high equivalent of theoretical plate value obtained is bigger than the theoretical value of the number plate.