Identifikasi Keragaman Gen ATP Binding Cassette (ABCG2|PstI) pada Sapi Friesian Holstein dengan Metode PCR-RFLP

Abstract

ATP Binding Cassette (ABCG2) has effects on milk production and composition. Reseptor which is encoded by the ABCG2 gene on alveoli epitel of mammary gland will bind its specific substrates, such as riboflavin, that are actively secreted, so milk will be rich of riboflavin. This study was aimed to identify the ABCG2 gene polymorphism in Holstein Friesian (HF) by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. This study used HF cattle by 224 heads from seven locations (BBIB Singasari, BIB Lembang, BPPTU Baturaden, BET Cipelang, BPPT-SP Cikole, KPSBU Pasir Kemis, and Cilumber). Data were analyzed for genotype frequency, allele frequency, and PIC value. Genotyping analysis resulted two alleles, namely A allele (292 bp) and C allele (268 bp, 24 bp); that resulted three genotypes, namely AA, AC, and CC. Analysis of genotype frequency resulted that AA homozygous was very highest (0.964) vs AC heterozygous (0.031) and CC homozygous (0.004); with allele frequency (A = 0.98; C = 0.02). PIC value ranged 0.000-0.189. This study concluded that ABCG2|PstI were polimorphic in 2 tested populations, the exception of monomorphic was in BBIB Singasari and BET Cipelang.