Karakteristik Keju Lunak Probiotik dengan Bahan Koagulan Kalsium Klorida pada Konsentrasi yang Berbeda

Abstract

Cheese is one of dairy products produce by using rennet as coagulant enzym. The use of rennet as coagulant has some problems such as limited availability and unguaranted halalness. Calcium chloride can be used as a substitution of milk coagulation process by rennet. The experiment was aimed to test the ability of calcium chloride salts as coagulant in the cheese-making process and to study the characteristics of the produced cheese by the addition of the probiotic bacteria. Concentration of calcium chloride used were 5%, 7.5% and 10% with the addition of probiotic bacteria Lactobacillus acidophilus IIA-2B4 and Bifidobacterium longum RRM-01. Statistical design used in this experiment were Randomized Block Design. The data obtained were analyzed with ANOVA and Tukey test was performed if significant defference among variables data was detected. Based on the MFFB and population of lactic acid bacteria, the produced cheese can be categorized as a soft cheese and also categorized as a probiotic food. The addition of calcium cloride at different consentrations did not effect significantly on the tested variables (viscosity, yield, nutrient composition, pH and total titrable acid, population of lactic acid bacteria, S, aureus, coliform and hedonic test) when compared with cheese coagulated by rennet as positive control, whereas calcium salt coagulation time showed highly significant difference (P<0.1) when compared with rennet, but did not differ significantly between the calcium salts. Thus the suggested level of addition of calcium chloride for cheese making is 5%.