Pengaruh Teknik Sterilisasi terhadap Keberhasilan Inisiasi Eksplan Paulownia (Paulownia elongata SY. Hu) secara In Vitro

Abstract

In vitro culture is used as one of the solutions in paulownia propagation. Contamination is a major problem in procedures micropropagation. This study examines the effect of sterilization treatment on the percentage of contamination, browning, and shoot induction paulownia. Sterilization techniques used in the study consisted of four treatments, namely A (Dhitane M-45 0.2 g/100 mL, Agrept 80 WP 0.2 g/100 mL, Bayclin 20%, 15%, 10%), B (Dhitane M-45 2 g/100 mL, Agrept 80 WP 2 g/100 mL, Bayclin 15%, 10%, 5%), C (Dhitane M-45 0.2 g/100 mL, Agrept 80 WP 0.2 g/100 mL, So Klin 20%, 15%, 10 %), and D (Dhitane M-45 2 g/100 mL, Agrept 80 WP 2 g/100 mL, So Klin 15%, 10%, 5%). Sterilization techniques give the same effect on the percentage of contamination, browning, and shoot induction paulownia. Contamination caused by bacteria and fungi is 93.96% and 11.5% respectively. Explants without contamination and browning shows the development of axillary buds. Some of explants produced callus as a response to highly endogenouse hormone. Low concentration of auxin can induced 100% callus formation at the stage of multiplication