Toksisitas dan Aktivitas Antioksidan Ekstrak Daun Pohon Penghasil Gaharu Hasil Inokulasi

Abstract

Gaharu leaves are expected to contain many secondary metabolic compounds as a response against fungal infection on gaharu-producing trees. This study aimed to determine the phytochemical components, cytotoxic and antioxidant activities of Aquilaria microcarpa and A. malaccensis leaves with and without fungal inoculation. Gaharu leaves were extracted by using methanol. The phytochemical assay of the crude extract revealed the presence of tannins, steroids, phenols, and flavonoids. The toxicity evaluation with brine shrimp showed that all samples were potencial as active ingredient, but only the juvenile leaves of A. microcarpa inoculated by fungi from Papua and Gorontalo had the potency as anticancer due to their LC50 value < 30 μg/mL. Free radical scavenging activity of the extracts against 2,2-diphenyl-1-picrylhydrazyl showed strong antioxidant activities with IC50 value of < 200 μg/mL. The highest antioxidant activity was exhibited by juvenile leaves of A.microcarpa inoculated by fungi from Timor and uninoculated mature leaves of A. malaccensis.