Isolasi dan Karakterisasi Protein Pupa Bombyx mori serta Aplikasinya pada Pembuatan Susu Bubuk Tinggi Protein

Abstract

Silkworm pupae (Bombyx mori) could be potentially developed as an alternative fortificant source for high protein powdered milk. The objectives of this research are 1) to isolate the silkworm pupae’s protein and to characterize the acquired silkworm pupae’s protein isolate (SPPI), 2) to discuss powdered milk’s characteristic affected by SPPI fortification and to find its best percentage fortification. The treatment used was fortification of SPPI into powdered milk in different levels as follows: 0%, 5%, 10%, 15%, and 20%. Defatting process decreased 60.87% fat content of powdered pupae. Modified protein isolation method produced SPPI with high purity protein, 81.84%. Protein quality of powdered milk increased after SPPI fortification and 20% SPPI fortification was selected as the best percentage producing high protein-claimed powdered milk with acceptable physical and chemial characteristic.

Pupa ulat sutera (Bombyx mori) memiliki potensi untuk dikembangkan sebagai sumber fortificant alternatif pada susu bubuk tinggi protein. Penelitian ini bertujuan: 1) mengisolasi protein pupa ulat sutera dan mengarakterisasi isolat protein pupa ulat sutera (IPPUS) yang dihasilkan, 2) menerangkan dampak fortifikasi IPPUS terhadap karakteristik susu bubuk dan mencari persentase terbaik fortifikasi IPPUS. Perlakuan yang digunakan ialah fortifikasi IPPUS ke dalam susu bubuk dengan persentase yang berbeda, yaitu 0%, 5%, 10%, 15%, dan 20%. Delipidisasi menurunkan 60.87% kandungan lemak tepung pupa sedangkan modifikasi pH isolasi protein menghasilkan kemurnian protein IPPUS yang tinggi, 81.84%. Fortifikasi IPPUS meningkatkan kualitas protein susu bubuk dan 20% IPPUS dipilih sebagai persentase terbaik dengan susu bubuk yang dihasilkan berklaim tinggi protein dan berkarakteristik fisik dan kimia masih layak.