Induksi Kalus dan Embrio Somatik Tanaman Jambu Biji Merah (Psidium guajava L.)

Abstract

The objective of the research was to get the best protocol for callus and somatic embryo induction of guava. The experiment was conducted at Tissue Culture Laboratory, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University from September 2012 until March 2013. The research was arranged into two experiments, that were callus induction and somatic embryo induction. The results showed that media with plant growth regulator composition treatments R2 (MS + 5 mg l-1 2.4-D), R4 (MS + 5 mg l-1 2.4-D + 1 mg l-1 BAP) and R5 (MS + 2.5 mg l-1 2.4-D + 2 mg l-1 2-iP) were the best treatments for inducting compact callus in 4 weeks after culture (WAC). In while, the friable callus can be induced at media with plant growth regulator composition treatment R6 (MS + 5 mg l-1 2.4-D + 2 mg l-1 2-iP). The primary somatic embryos can be induced at the treatment KR1E4 (callus from MS + 2.5 mg l-1 2.4-D was moved into MS + 1 mg l-1 2.4-D + 2 mg l-1 2-iP) and the treatment KR6E4 (callus from MS + 5 mg l-1 2.4-D + 2 mg l-1 2-iP was moved into MS + 1 mg l-1 2.4-D + 2 mg l-1 2-iP) in 12 and 16 WAC for each treatment.