Respon Embrio Somatik Bawang Merah Kultivar Ilokos terhadap Polietilen Glikol 6000 dalam Kultur In Vitro

Abstract

The aims of the experiment was studied the responce somatic embryo to polyethilene glicol 6000 (PEG-6000) as in vitro drought selection agent in Allium ascalonicum L. cultivar Ilokos. The experiment held at tissue culture laboratory, Agronomy and Horticulture Department, Faculty of Agriculture, Bogor Agricultural University, from August to December 2012. Experiment used Completely Randomized Design, single factor of PEG-6000. The factor was concentration of PEG-6000 consisted of 0%, 5%, 10%, and 20%, each treatment repeated 13 times. The results showed that 100% initial explant were induced become embriogenic callus in 2.4-D 2 mg l-1 medium in third weeks after aplication. Good effect was obtained to complete somatic embryo growth from BAP 10 mg l-1 with total number of somatic embrio formed was 454 embryos, but it could not encouraged plant regenaration. Generally, somatic embryos was supposed still survive for two weeks in 5%-20% PEG-6000 selection mediums.