Penentuan Kuersetin dalam Bawang Dayak (Eleutherine palmifolia) dengan Kromatografi Cair Kinerja Tinggi

Abstract

Eleutherine palmifolia known as bawang dayak or bawang sabrang is widely used as herbal medicine to treat various diseases, including diabetes, hypertension and lowering cholesterol. The objectives of this research are to determine content of quercetin in bawang dayak and to analyze the performance of determination method of quercetin in bawang dayak using high-performance liquid chromatography (HPLC). To ensure that HPLC method can be used for the intended purpose then the method has to be validated. Parameters determined on method validation were linearity, limit of detection, limit of quantification, and precision. The linearity in the concentrations of 0.5, 2.5, 10, and 20 mg/L showed correlation coefficient of 0.9992. The limit of detection was 0.0482 mg/L and the limit of quantification was 0.1460 mg/L. The precision of this method was 13.41%. The average concentration quercetin in bawang dayak was 0.2943%. The number of quercetin that is high enough make bawang dayak potential as a medicine.