Evaluation of galohgor effect on wound healing process of female rats (Rattus norvegicus) sprague dawley strain

Abstract

Galohgor, a kind of herbal medicine that also known as a traditional food supplement, contains nutrient, especially beta-caroten and bioactive compound. This herbal medicine affects the uterine involution and wound healing process after delivery. The proliferative phase of wound healing is characterized by angiogenesis, collagen deposition, granulation tissue formation, epithelialization, and wound contraction. Re-epithelialization of the epidermis occurs, in which epithelial cells proliferate and providing cover for the new tissue. The objective of this study is to elaborate the effect of galohgor supplementation on beta-caroten, retinol, zinc and iron level on blood, and wound healing process. Twenty four female mice 2 months old, 200 g weight, were divided into 2 groups, i.e. group without jamu as control and group with jamu supplementation. Each group was divided into 2 subgroups, i.e. group were given dorsal cutaneous injury diameter 0.5 cm and without dorsal cutaneous injury. Each of the subgroups composed of 2 mice based on the period of termination, i.e.: 3rd, 7th and 12th day after wounded. The blood and skin histological samples were collected on each termination days. The data were analyzed by independent samples t-test. The results showed that galohgor suplemented group had relatively higher serum β-carotene and lower serum retinol than control groups. Blood performance, zinc and iron level were not different significantly between groups. According to its limphocytes counting, re-ephitelization, angiogenesis, and colagen deposition of immunohistology of wound skin, galohgor supplemented group was healed faster than control group. The result showed that the herbal medicines Galohgor is hastened wound healing process.