Kualitas Spermatozoa Kauda Epididimis Domba Masa Tumbuh yang Diberi Ekstrak Etanol Daun Katuk pada Penyimpanan 4ºC

Abstract

Katuk is one of plant that has active compounds which can stimulate reproductive hormone. The purpose of this research was to know the effect of extract katuk leaves in ethanol feed to growth ram on cauda epididymal sperm characteristic. This research is done on six growth local rams around five months old which was divided into two groups, EtOH group and control group. Ram were feed 1500 mg/day of extract katuk leaves in ethanol for two months, where in control group were feed only by placebo. The epididymis were collected after slaughter and the sperm were collected. Collected sperm were analyzed for several sperm characteristic such as concentration, motility, viability, membrane integrity, and abnormality during stored for five days in refrigerator. Sperm concentration and epididymal weight were checked on first day. The result of this research showed there were no significant difference in the sperm concentration in both treatment. Sperm motility, viability, membrane integrity, and abnormality was decreased day by day. However, no significance difference in sperm characteristic in both treatment during storage for five days. The sperm motility was no detected on the day four. Interestingly, the viabilitywere still high and detected until the end of research.