Pengaruh Nikotin pada Gambaran Mikroskopis Pankreas Monyet Ekor Panjang (Macaca fascicularis) yang Diberi Pakan Berenergi Tinggi

Abstract

Nicotine, in a low dosage, is expected to be used for health problems therapy such as obesity, hypertension, diabetes, heart disease, and other metabolic syndrome diseases. The aim of this study was to evaluate the microscopic changes of the pancreas in long tailed macaque which fed a high-energy diet with an oral intervention of nicotine. Twelve monkeys were devided into two groups based on nicotine and non nicotine treatment. Group with nicotine consisted of ten monkeys were further divided into two groups based on the type of diet. Each group consisted of five monkeys. The main content of diet A was tallow and diet B was commercial feed (monkey chow). Liquid nicotine intervention with a dosage of 0.75 mg/kg body weight were given every twelve hours for three months on both groups. While the non nicotine treatment was a negative control consisted of two monkeys were given diet B. All monkeys were under went to be euthanized by initially anesthetizing using ketamine HCl 10 mg/kg, intramuscularly. Microscopic samples were prepared based on the three pancreatic areas, caput, corpus, and cauda. They were stained with haematoxylin-eosin (HE) for standard staining, and Congo red to evaluated amyloid accumulation on the pancreatic islet. Data were analyzed descriptively and the microscopic findings showed that there were hydropic and lipid degeneration in all treatment and control groups as well as mild inflammation in one of the animals and no evidence of amyloid accumulation. As the conclution that nicotine oral administration on monkeys which fed high energy diets gave no difference in pancreatic microscopic finding amongs the groups