Perbandingan Metode Hidrolisis dalam Aktivitas Pembentukan Asam Lemak Rantai Pendek pada Pati Taktercerna Tipe 3

Abstract

Resistant starch is a fraction of starch not absorbed by the digestive system in healthy individuals, one of which is RS3, a retrograded starch and has similarities with digestibility process of starch in human body. This study aimed to evaluate the ability of Eubacterium rectale and Clostrydium butyricum consortium in producing short chain fatty acids (SCFA), namely acetic, propionic, and butyric acids from RS3 under 3 different hydrolysis methods. Yield of resistant starch from hydrolysis using 2 N HCl , α-amylase, and pullulanase were 6.6%, 2.3%, and 48.1%, respectively, with the degree of polymerization were 15, 18, and 25 residues, respectively, meaning that they meet the requirement as RS3. This experiment resulted an average of butyrate:propionate:acetate which was 130:60:50, which was close to an ideal SCFA metabolism. Fatty acids produced using RCM (reinforced clostridial medium) medium was similar to that of using PYG (pepton yeast glucose) medium, although the levels were significantly different among the hydrolysis methods used