Pengaruh komposisi media dalam pertumbuhan protocorm like bodies, planlet, dan aklimatisasi phalaenopsis amabilis

Abstract

Phalaenopsis amabilis, known as ‘anggrek bulan’, is one of the most popular kind of orchids and was inaugurated as one of the national flower as 'Puspa Pesona'. The purpose of this study was to determine and study the effects of various tissue culture media composition on Protocorm Like Bodies (PLBs) multiplication, growth of plantlets, and acclimatization of Phalaenopsis amabilis. Basic medium used was a half strengh of Murashige and Skoog (1/2MS) and Hyponex (Hyp) with additional coconut water (CW), benzylaminopurine (BAP), and chitosan. In PLBs multiplication experiments, ½ MS + 15% CW medium produced the highest percentage of survival rate. Treatment with ½ MS + 15% CW medium and Hyp. 2 g/l + 15% CW + 2.5 ppm chitosan medium gave the best effect on the fresh weight of plantlets. The composition of the culture medium significantly affect the fresh weight and length of root on 8-WAP(week after planting) in the acclimatization stage.