Kualitas Semen Domba Garut dengan Pemberian Pakan Limbah Tauge dan Indigofera sp pada Pengencer Tris Kuning Telur.

Abstract

Garut ram is one of good comodity of the livestock, but still traditional management systems. This is a delimiter in garut ram heir superior quality. Semen preservation in several extender can be as choice to maintain genetic superior of garut ram. Semen quality itself affected by the nutrient factors contained in the waste bean sprouts and Indigofera sp such as protein and vitamin E, which is capable of affecting the quality of spermatozoa in garut ram. The research was aimed to study the influence of feeding with 30% waste bean sprouts and 30% Indigofera sp on sperm quality in the tris egg yolk extender. Semen sample were collected from 4 garut male with age around 11 months were two garut ram was fed with limbah tauge and two garut ram was fed with Indigofera sp. Collected semen were diluted in tris egg yolk extender and stored at refrigerator 5 oC until fifth days. Parameter of semen quality was analyzed as follow: for the colour, consistency, pH, volume semen and sperm mass movement were expressed with descriptively analysis. Concentration and cytoplasmic droplet were tested by T-Test analysis. Sperm motility, sperm viability, integrity of sperm plasma membrane and sperm secondary abnormality were analyzed using Completely Randomized Design Factorial (2x5). The first factor was fed (waste bean sprouts and Indigofera sp) and the second factor was storage time (day 1st,2st,3st,4st and 5st). Data was analyzed by Analysis of Variance (ANOVA) and continued with Duncan test to observed the different between treatments. Result of the experiment showed descriptively: colour, concistency and semen pH in all treatment have same quality. Semen volume were collected from garut ram with fed waste bean sprouts better than with fed Indigofera sp, whereas sperm mass movement with fed Indigofera sp better than with fed waste bean sprouts. T-test analyzed showed all treatment were not significant different (P>0,05) to sperm concentration and cytoplasmic droplet. Analysis of Variance showed sperm motility in all treatment were not significant different (P>0,05) whereas in first and second days storage significant better than after third storage (>40%). Such as with sperm viability and integrity of sperm plasma membrane were significant maintaint until fourth storage with percentage (>60% and >50%). Percentage sperm secondary abnormality in treatment with fed Indigofera sp significant better than treatment with fed waste bean sprouts. It can be summarized that all treatment were not influence to sperm quality garut ram 11 years old, but storage time influential to sperm motility, sperm viability, and integrity of sperm plasma membrane. It is suggested to do further research the influence of both feed on adult ram.