Perbanyakan dan penginduksian keragaman somaklon pada tanaman dianthus Caryophyllus Linn melalui kultur in vitro

Abstract

Penelitian ini bertujuan pertama untuk mendapatkan metode perbanyakan in vitro Dianthus caryophyllus Linn. yang menghasilkan planlet tegar, mampu tumbuh dan berkembang menjadi tanaman dewasa dan berproduksi (berbunga), dengan menggunakan retardan. Tujuan kedua mendapatkan metode untuk memperoleh keragaman somaklon melalui tehnik in vitro baik tanpa radiasi maupun dengan radiasi.

The objectives of the studies were: ( 1) to develop dependable in vitro propagation method in obtaining viger­ous planlets by using retardant, (2) to develop method of obtaining detectable in vitro variation both spontaneous as well as using irradiation technique. Research was conducted in series of experiments, ( 1) three preliminary experiments (cv "Scania", "Calypso" and "White candy") , ( 2} two experiments on propagation and :somaclonal variation (cv "Scania" and "Pink Spray"), one ,experiment on aclimatitation (cv "Pink Spray"), and (3) two ,experiments on callus embryogenesis (cv "Scania", "Pink Spray", "Red Corso", and "Lanras"). The purpose of the preliminary field experiment was to study the growth pat­tern of cutting upto flowering. Lab experiments were de­signed to study the effects of auxin IAA, cytokinin BAP and kinetin on shoot formation, and concentration level of :2, 4-D, cytokinin BAP and kinentin on callus induction.