Antibacterial Activity of Fractionated Extract of Sansevieria trifasciata

Abstract

Sansevieria trifasciata is an indigenous plant of South Africa used in herbal medicine for the treatment of various infectious diseases. The purpose of this study is to evaluate potency of S. trifasciata extracts through in vitro antibacterial test against Grampositive (Staphylococcus aureus) bacteria and Gram-negative (Escherichia coli) bacteria on Mueller-Hinton medium. The plant sample were obtained from research garden at Lodaya Campus, Bogor Agricultural University. The result indicated that methanol crude extract at concentration 10000, 5000, 2500, and 1250 ppm showed no activity against the tested bacteria. Methanol crude extract was fractionated using coloumn chromatography with gradient method. Methanol and chloroform were used as eluent with the best ratio of 9:1. The fractionation yielded 10 fractions. The fractions were tested at concentration 40000, 20000, and 10000 ppm. The antibacterial assay showed there were 2 active fractions for both of the tested bacteria, 5 active fractions against only Gram-positive (S. aureus) and 3 non-active fractions.