Pembersihan Isi Sel Akar dan Jenis Warna Tinta untuk Deteksi Cendawan Mikoriza Arbuskula

Abstract

Arbuscular mycorrhizal (AM) fungi is a symbiotic mutualistic fungi association with plant roots. Staining technique to detect AM fungi usually used hazardous chemical. The ink stain and vinegar were used as alternative technique to replace trypan blue and lactic acid in root staining method. This study aimed to determine time of clearing of root cell contents and types of ink stain to produced the best AM fungal structures within the root observed under microscope. Pueraria javanica root from 6 pot cultures were cut into 1 cm long. They were cleared and stained using 4 types of stains. Twenty stained roots were taken randomly from each stain types, and observed. Root clearing process for 20 minutes showed the best result. Only shaeffer black ink and trypan blue produced clear structure of external hyphae, internal hyphae, vesicles, and arbuscule. Arbuscular structure only stained by shaeffer black ink and trypan blue. This indicated that shaeffer black ink could be used as an alternative stain to detect AM fungi within the P. javanica root.