Exploration and identification of anti-fungal compounds for some tropical woods

Abstract

Widely used wood preservation is made from synthetic chemistry, where the raw material is non-renewable and environmental unfriendly. One solution is to look for alternative preservations using certain extractive substances from a durable tropical wood as a wood preservative. The main objectives of this study were to determine the potential extractive substances in woods of kupa (Syzygium polycephalum (Mig)), pelawan (Tristaniopsis whiteana (Griff)), palepek baringin (Shorea laevis Ridl), and mahalilis (Palaquium sp.), to evaluate the use of extractive substances as anti-fungal wood decay Schizophyllum commune Fries and Pleurotus ostreatus, and to identify the structure of anti-fungal compound. The maceration extraction method used methanol solvent, and multilevel fractionation used n-hexane, chloroform, ethyl acetate and butanol solvents. The extracts, subsequently, were carried out fungus S. commune and P. ostreatus the tests, and CCB preservative was used as negative control. The result showed that the highest extractive content was in S. laevis, followed by Palaquium sp., T. whiteana and S. polycephalum. Moreover, the results of column chromatography showed that ethyl acetate fraction of S. polycephalum was obtained 8 (G.1-G.8) compounds, the fraction of chloroform of T. whiteana was obtained 8 (PL.1-PL.8) compounds, the fraction of butanol of S. laevis was obtained 16 (PB.1-PB.16) compounds and the fractions of chloroform of Palaquium sp. was obtained 5 (M.1-M.5). Based on the maceration extract, fractionation and column chromatography, all the wood types were capable of inhibiting the growth of the fungus S. commune and P. ostreatus. Structure identifications with LC-MS, 1H and 13C NMR anti-fungal compound for G.2, Pl.3, PB.1.1, and M.2 were 3-O-glucosyl-3’,4’,5-trihydroxyflavonol, Heptanoic acid, 2,3-dihydroxyoctadecanoic acid and 2,3-dihydroxypropylpentadecanoate, respectively .