Effectivity of Immersion Thyroxine and Recombinant Growth Hormone on Variability of Sutchi Catfish Fry

Abstract

This research was conducted to determine the effectiveness of the hormone thyroxine (T4) and recombinant growth hormone (rGH) through immersion method on variability of sutchi catfish fry. This research was using factorial completely randomized design with two factor is T4 doses (0, 0.01, 0.05, and 0.10 mg/L) and rGH doses (0 and 10 mg/L). All treatment was repeated three times. Catfish fry used had an average total length of 3.2±0.2 cm and body weight 0.27±0.07 g. Frys immersed in a solution of hormones for 1 hour, then were reared for 15 days and fed pellet on 10% of the fish biomass. The results showed that any given hormone treatment did not impact significantly different (P>0.05) on growth (T1 4.7 cm; T2 4.7 cm; T3 4.7 cm; GT1 4.7 cm; GT2 4.8 cm; GT3 4.8 cm; rGH 4.6 cm) and survival rate (T1 97.73%; T2 97.33%; T3 97.07%; GT1 96.27%; GT2 95.47%; GT3 98.13%; rGH 97.60%) compared to the control (4.6 cm; 98%). Treatments GT2 (145%) and GT3 (147%) showed feed efficiency significantly different (P<0.05) compared to the control (141%), T1 (135%), T2 (134%), T3 (130%), GT1 (140%), and rGH (131%). Thus, a compound of the hormone (T4 and rGH) give the best feed efficiency.

Penelitian ini dilakukan untuk mengetahui efektivitas hormon tiroksin (T4) dan hormon pertumbuhan rekombinan (rGH) melalui metode perendaman terhadap keragaan benih ikan patin. Penelitian ini menggunakan rancangan acak lengkap faktorial dengan dua faktor yaitu dosis T4 (0, 0.01, 0.05, dan 0.10 mg/L) dan dosis rGH (0 dan 10 mg/L). Semua perlakuan diulang tiga kali. Benih ikan patin yang digunakan memiliki rata-rata panjang total 3.2±0.2 cm dan bobot tubuh 0.27±0.07 g. Benih direndam dalam larutan hormon selama 1 jam, kemudian dipelihara selama 15 hari dan diberi pakan buatan sebesar 10% dari biomassa ikan. Hasil penelitian menunjukkan bahwa setiap perlakuan hormon yang diberikan tidak memberikan pengaruh yang berbeda nyata (P>0.05) terhadap pertumbuhan (T1 4.7 cm; T2 4.7 cm; T3 4.7 cm; GT1 4.7 cm; GT2 4.8 cm; GT3 4.8 cm; rGH 4.6 cm) dan tingkat kelangsungan hidup (T1 97.73%; T2 97.33%; T3 97.07%; GT1 96.27%; GT2 95.47%; GT3 98.13%; rGH 97.60%) dibandingkan kontrol (4.6 cm; 98%). Perlakuan GT2 (145%) dan GT3 (147%) menunjukkan efisiensi pakan yang berbeda nyata (P<0.05) dibandingkan kontrol (141%), T1 (135%), T2 (134%), T3 (130%), GT1 (140%), dan rGH (131%). Dengan demikian, pemberian hormon campuran (T4 dan rGH) memberikan efisiensi pakan yang terbaik.