Growth and survival of white shrimp post-larvae administered recombinant growth hormone by different immersion time

Abstract

This research was conducted to determine the optimum immersion time of recombinant giant grouper hormone (rElGH) at a dose of 15 mg/L that generates highest growth of white shrimp post-larvae at PL-2 phase. This research consisted of five treatments and threeplicates. The treatments were immersion time for 1, 2 and 3 hours. A total of 1,500 PL-2 shrimp were bath immersed in a plastic packing containing 1-L sea water, 15 mg/L rElGH and 0.01% bovine serumalbumin (BSA). Two kinds of control was performed, namely it was without any treatment (control), and immersed in water containing 0.01% BSA and total protein of Escherichia coli without rElGH (pCold control). PL were further maintained for 18 days in the aquarium, fed nauplii Artemia and flake commercial diet, 7 times feeding; 5 times by flake and 2 times by nauplii Artemia, at satiation. The results showed that the highest of the average biomass (36,289.87±1459,56 mg), specific growth (29,81±0,87 g%), and body length (20.08±0,42 mm) were obtained in 3 hours immersion treatment (p<0.05). Biomass of PL in 3 hours immersion treatment was approximately 66.0% higher compared to the control (21,872.19±2529,40 mg). Survival of shrimp in all treatment and control were similar (p>0.05). Thus, the bath immersion time of post-larvae for 3 hours in water containing 15 mg/L rElGH could be used to increase growth, and the application of this technology can be useful to increase aquaculture production.

Penelitian ini dilakukan untuk menentukan lama waktu perendaman hormon pertumbuhan rekombinan ikan kerapu kertang (rElGH) dosis 15 mg/L yang menghasilkan pertumbuhan tertinggi pada post-larva udang vaname fase PL-2. Penelitian ini menggunakan 5 perlakuan dengan 3 ulangan. Perlakuan yang diberikan adalah lama perendaman 1 jam, 2 jam, dan 3 jam. Sebanyak 1500 ekor PL-2 direndam dalam kantong plastik kemasan berisi air laut mengandung rElGH dan serum albumin sapi (BSA) 0,01%. Dua jenis kontrol dibuat, yakni udang vaname PL-2 tidak diberi perlakuan (kontrol), dan direndam dalam air mengandung BSA 0,01% dan protein Escherichia coli tanpa rElGH (kontrol pCold). Selanjutnya, udang dipelihara selama 18 hari di dalam akuarium, dan diberi pakan naupli Artemia dan pakan komersial flake hingga kenyang sebanyak 7 kali; 5 kali pakan flake dan 2 kali pakan Artemia per hari. Hasil penelitian menunjukkan bahwa rerata biomassa (36.289,87±1459,56 mg), pertumbuhan spesifik (29,81±0,87 g%), dan panjang tubuh (20,08±0,42 mm) tertinggi (p<0,05) diperoleh pada perlakuan perendaman selama 3 jam. Biomassa udang perlakuan perendaman selama 3 jam lebih tinggi sekitar 66,0% dibandingkan dengan kontrol (21.872,20±2529,40 mg). Kelangsungan hidup udang perlakuan hidup udang yang direndam dengan rGH tidak berbeda nyata (p>0,05) dengan kontrol dan kontrol pCold. Dengan demikian, perendaman post-larva udang vaname selama 3 jam dalam air mengandung rElGH 15 mg/L dapat meningkatkan pertumbuhan, dan aplikasi teknologi ini dapat berguna untuk meningkatkan produksi budidaya udang vaname.