Penapisan Bakteri Lipolitik asal Fruktosfer Kelapa Sawit

Abstract

Lipase (EC 3.1.1.3, glycerol ester hidrolase) is defined as carboxylesterase that catalyzes the hydrolysis of long chain asilgliserol. Lipase produced by lipolytic bacteria that grow well in substrates containing oils such as oil palm fruit. The purpose of this study was to screen lipolytic bacteria from oil palm fructosphere and to characterize optimal pH and temperature of lipase enzyme from selected isolates. Seven isolates of lipolytic bacteria with different morphology were qualitatively screened from oil palm fructosphere using Rhodamin B media. Bacteria were isolated from the exocarp and mesocarp of ripe oil palm fruits. Lipolytic activity each isolates were measured and selected based on their activity. Three isolates possessed the best activity with result as follows EP2.22 (0.912 U/mg), F2 (0.792 U/mg), and F6 (0.650 U/mg). These isolates showed high activity at various range of pH and temperature. Isolate EP2.22 showed optimum pH at 7.5 and optimum temperature at either 40 oC or 70 oC. Isolate F2 showed optimum pH 8.5 and optimum temperature 70 oC. Isolate F6 showed optimum pH 8.5 and optimum temperature at 30 oC and 70 oC. Two peaks of high activity due to the use of crude enzyme extract. 16S rRNA gene sequencing indicated that isolates EP2.22, F2, and F6 closely related to Staphylococcus klosii Enterobacter sp., and Buttiauxella izardii.