Identifikasi Bakteri dan Cacing Parasitik pada Insang dan Saluran Pencernaan Ikan Nila Hitam (Oreochromis niloticus)

Abstract

The objectives of this research were to identify parasitic worms and bacteria in gills and digestive tract of nile tilapia (Oreochromis niloticus). A group of 10 fishes of nile tilapia were used, each gills and digestive tract was collected. The parasitic worms were colored with KOH and clove oil for semi-permanent staining, and Semichon’s Acetocarmine for permanent staining. The isolated bacteria were identified using Gram staining, Triple Sugar Iron Agar, citrate agar, urea agar, indole, and cabohydrate fermentations. The result showed that there were three kind of parasitic worms in gills of nile tilapia, Dactylogyridae, Dactylogyrus sp., and Pseudodactylogyrus sp. The total amount of Dactylogyridae is 8 worms, Dactylogyrus sp. is 72 worms, and Pseudodactylogyrus is 24 worms. The bacteria were identified and the result showed that Aeromonas sp., Bacillus sp., Escherichia coli, Edwardsiella tarda, Klbesiella pneumoniae, Pasteurella sp., Staphylococcus epidermidis, and Vibrio parahaemolyticus were come from gills. Aeromonas sp., Bacillus sp., Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Staphylococcus aerus, Staphylococcus epidermidis, Streptococcus sp., and Vibrio parahaemolyticus were come from digestive tract. The relation between the parasitic worms and the bacteria did not significanty obvious. The parasitic worms might predispose the secondary infection caused by bacteria or might be the opposite.