Keanekaragaman genetik rayap tanah genus Coptotermes (Isoptera: Rhinitermitidae) di pulau Jawa

Abstract

Subterranean termite Coptotermes spp. has been known as the most economically important structural pest in Indonesia. Due to morphological ambiguity, traditional identification of Coptotermes spp. has always been difficult and unreliable. Through molecular diagnostic method, a study was conducted to determine genetic variation of Coptotermes spp. occuring in Java Island. Ternite specimens were collected from Banten 1, Banten 2, DKI 1, DKI 2, Jabar 1, Jabar 2, Jateng 1, Jateng 2, Yogya 1, Yogya 2, Jatim 1, and Jatim 2. The method for identification was PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis using four restriction enxymes each of which was applied to CO II amplicon for all the Coptotermes spp. being analyzed. The results showed the existence of two species of Coptotermes in Java which are different from both Coptotermes gestroi and Coptotermes formosanus.