Controlled Deterioration Test untuk Menguji Ketahanan Benih Kacang Hijau (Phaseolus radiatus L.) terhadap Kondisi Cekaman Kekeringan.

Abstract

The research was conducted from February to April 2012 at the Seed Science and Technology Laboratory, Agronomy and Horticulture Department, Bogor Agricultural University. This research consist of three experiment. The first experiment was to determine the tolerance of five varieties of mungbean seeds to drought using PEG 6000 with different levels of osmotic pressure ( 0, -0.5, -1, -2, and -3 bar). The second experiment, determining the seed vigor of five mungbean seed lots using controlled deterioration test (CDT) with condition of temperature was 45°C, the seed moisture content were 20%, 22%, 24%, 26% and the deterioration time were 0 h, 24 h, 48h, 72h. The experimental design that used was randomized complete block design with two factor. The third experiment was to determine the relationship between the results of first experiment with the second experiment results. The third experiment also could show the effectiveness of the CDT method in testing the resistance of mungbean seeds against drought stress. Based on the observations of the variable results, it was obtained that the -1 bar PEG 6000 had capability of selecting seeds that were resistant and not resistant to drought. The result of the second experiment was CDT conditions that can be used to test for seed vigor were 20%/48 hours and 22%/24 hours. Correlation analysis between the variables in the -1 bar PEG 6000 with CDT on two conditions CDT (20%/48 hours and 22%/24 hours) showed significant correlation to the normal germination percent(r= 0.61 and 0.58) and speed germination(r= 0.62 and 0.59 ).