Determination of anti-hyperuricemic extract of celery, sidaguri, and tempuyung by in vitro method

Abstract

Gout is certain a consequence indicated by accumulation of monosodium urate in body causing gout arthritis. Celery (Apium graveolens), sidaguri (Sida rhombifolia), and tempuyung (Sonchus arvensis) are Indonesian native medicinal plants to reduce uric acid contents in blood. Based on their active compounds, they may inhibit xanthine oxidase while the enzyme itself could convert xanthine into uric acid in blood. The objective of this study was to determine the inhibition of single extract and mixture of extracts of celery, sidaguri, and tempuyung toward xanthine oxidase by in vitro method Inhibition of xanthine oxidase by in vitro was examined at the optimum condition (20 oC of incubation temperature, pH 7.5, 0.1 unit/ml of xanthine oxidase concentration, and 0.7 mM of xanthine concentration) was compared with allopurinol as positive control. The results indicated that single extract of 300 ppm of sidaguri, 1200 ppm of celery, and 300 ppm of tempuyung that have greatest inhibition of 42.41%, 67.23 %, and 47.51%, respectively. In addition, the combined extract of sidaguri, celery, and tempuyung with certain ratio showed in vitro inhibition of 71.62 %.