Isolasi dan Uji Antimikrob Metabolit Sekunder Ekstrak Kultur Jamur Endofit AFKR-5 dari Tumbuhan Akar Kuning (Arcangelisia flava (L) Merr)

Abstract

Endophytic fungi associated with medicinal plant were known to produce bioactive secondary metabolites similar to its host plant. This study aimed to obtain bioactive secondary metabolites as antimicrobial from endophytic fungi AFKR-5 associated with akar kuning plant from Bogor Botanical Garden. Methanol fraction of ethyl acetate extract from AFKR-5 culture in potato dextrose broth medium was capable to produce the bioactive secondary metabolites F3.4 (10.375 mg/L) with retention factor of 0.30. Antimicrobial activity test using Kirby-Bauer disc diffusion method showed that F3.4 was a broad antimicrobial spectrum on 3 pathogenic microbes, namely Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli, and Candida albicans mold. The determination of minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were carried out with liquid microdilution method. F3.4 was the most potential with MIC value of 16 μg/mL against C. albicans, twice as stronger than the commercial antifungal Nystatin which was only fungistatic with MIC value of 32 μg/mL. F3.4 was fungicidal with MFC value of 32 μg/mL against C. albicans, so that it is potential to be developed as antimicrobial, especially antifungal.