Karakteristik Spermatozoa Domba Selama Proses Pembekuan dengan Medium Pengencer yang Ditambahkan Glutation

Abstract

after freezing evaluated. Motility of spermatozoa in 3 mM 6 hours after thawing, higher than the control and 1 mM (p<0.05). Plasma membrane integrity of spermatozoa immediately after semen dilution and after diequilbration did not differ among treatments (p>0.05). Plasma membrane integrity immediately after thawing in the 1 mM GSH group higher than 3 mM GSH group (p<0.05). Three hours after thawing, plasma membrane integrity of spermatozoa in the 3 mM GSH group was higher than control group and 1 mM GSH groups (p<0.05), but 6 hours after thawing the integrity did not differ among treatment groups (p>0.05). Olthough there are differences in sperm viability among treatment groups immediately after semen dilution (p<0.05), but there were no significant differences in the viability of spermatozoa between the treatment groups after the equilibration and after thawing (p>0.05). No significant difference was found in the morphology of spermatozoa among the group (p>0.05). The addition of 3 mM glutathione to the freezing extender affected the motility of spermatozoa after 6 hours post-thawing and the plasma membrane integrity intact after 3 hours post-thawing.