Pemanfaatan Tris Sari Kedelai Sebagai Bahan Pengencer Semen Cair Kambing Peranakan Etawah.

Abstract

This research was aimed to examine and find the best concentrations of soybean extract in Tris buffer (TS) for Etawah Cross Breed (ECB) semen extender. Three sexually mature and healthy ECB buck were used as a source of semen. The semen was collected using an artificial vagina and evaluate macro- and microscopically. Ejaculate having > 70% sperm motility, > 2500x106ml-1 sperm concentration and < 15% abnormal sperm were individually processed. Each ejaculate divided into five tubes. Four tubes were mixed with 2.50% TS (TS2.50), 3.75% TS (TS3.75), 5.00% TS (TS5.00), and 6.25% TS (TS6.25), one other tubes was diluted with Tris egg yolk extender (TEY) with the concentration of spermatozoa was 50x106ml-1. The semen stored at 3-5oC and observed its sperm motility and viability every 12 hours until 72 hours of storage. After 72 hours of storage result showed that TEY was the best sperm extender (p<0.05) on the sperm motility as well as sperm viability compared to TS in all concentration. TS2.50 (58.33 ± 2.50%) and TS3.75 (50.56 ± 4.46%) was the best TS concentration compared to TS5.00 or TS6.25. For artificial insemination purpose, sperm extender in TEY, TS2.50 and TS3.75 can be used until 72 hour of storage.