Kemiripan Genetik Kappaphycus alvarezii Asal Beberapa Daerah di Indonesia.

Abstract

Red algae Kappaphycus alvarezii produced high economic value karageenan and used in the many industries such as pharmaceutical industry, food industry, stabilizer, and cosmetics. Recently, many cultivations of K. alvarezii has been done in Indonesian seas, such as in sea region Teluk Banten, North seashore of Java, Sulawesi, Bali, Lombok and the other seas area in Indonesia. However, K. alvarezii is one that easily infected by ice-ice disease. Genetic improvement is indispensable to increase productivity and genetic diversity needed on it. Genetic diversity analysis can be seen from genetic similarity. Genetic similarity analysis of K. alvarezii have not been done yet until recent time. One method to analyse genetic similarity is Amplified Fragment Length Polymorphism (AFLP). This study aimed to determine the similarity of six normal K. alvarezii and two samples infected by ice-ice disease collected from Research Group of Naval and Fishery (BRKP) Maros used AFLP with PstI and MseI as restriction enzyme. AFLP with P11 as forward primer and M48, M49, M50 as reverse primer treated to Takalar (TKL), Bone (BNE), Gorontalo (GRL), Tambalang (TMB), Mataram (MTR), Kendari (KND), Takalar ice (TKL+) and Mataram ice (MTR+) amplified 519 fragments in 122 loci in the size of 50 - ~370 bp. Genetic similarity of eight samples based on AFLP product showed high result with similarity coefficient range from 0.7951-0.9210. Genetic similarity of ice-ice infected algae was decrease compared to the normal samples. The similarity between TKL-TKL+ was 0.8176 and MTRMTR+ was 0.8033. The highest similarity was between TKL and TMB, then the lowest was between MTR and TKL+