Identifikasi Keragaman Gen Follicle Stimulating Hormone Receptor (FSHR|AluI) pada Spesies Sapi Bos javanicus, Bos taurus, dan Bos indicus dengan Metode PCR-RFLP.

Abstract

Follicle Stimulating Hormone Receptor (FSHR) gene is a very important in reproduction trait. FSHR is required by Follicle Stimulating Hormone (FSH) to carry out on target tissues. The purpose of this study was to identify polymorphism of the Follicle Stimulating Hormone Receptor gene in Bos javanicus, Bos taurus, and Bos indicus Cattle. Blood samples used 207 samples consisting of Bos javanicus (Bali Cattle) 59 heads, Bos taurus 137 heads (Limousin 57 heads, Simmental 50 heads, Angus 6 heads, and Holstein Friesian (FH) 24 heads), and Bos indicus 11 heads (Brahman 9 heads and Ongole 2 heads). The polymorphism site analysis from digestion with AluI restriction enzyme allowed the genotype identification. AluI restriction enzyme cut the base position at AG|CT in 193 and 243 bp. Genotyping FSHR gene produced two alleles, namely C and G alleles. These allele produced three genotypes respectively, there are CC (243 and 63 bp), GG (193, 63, and 50 bp), and CG (243, 193, 63, and 50 bp). The highest G allele frequency was found in Bos javanicus (Bali cattle), but the lowest in Bos taurus. The highest C allele frecuency was found in Bos taurus. The value of heterozigosity was the highest in Bos indicus and the lowest in Bos javanicus. The result of this study showed that the allele of FSHR gene in Bos taurus and Bos indicus were polymorphic, while in Bos javanicus was monomorphic.