Longivitas dan Viabilitas Spermatozoa Sapi Friesian Holstein, Simmental, dan Brahman dalam Semen Beku Menggunakan Pengencer Skim.

Abstract

Indonesia had two artificial insemination centre and more than 17 regional artificial insemination centre. Before distributed to farmers, the quality of frozen semen has to be laboratory tested periodically. According to the Indonesian National Standard the parameters assessed in the laboratory are sperm motility and individual scoring. The aim of this study was to test the sperm motility, viability and longevity of frozen semen from three cow breeds which are FH, Simmental and Brahman produced by one of the regional artificial insemination centers and to fine out the relationship between sperm motility and its viability among breed. Results showed that post thawing motility of the FH bull was 50.00±0.00% which was significantly higher than Simmental 43.50±2.42% or Brahman 43.33±2.50% bull. A decrease in motility and viability showed an association motility with the percentage of live spermatozoa. They were no differences in sperm longevity among breeds. The motility of all samples decreased and reached 0% motility in 5 hours. The decrease in sperm motility and viability is caused by the decrease in energy sources and the accumulation of toxic waste of metabolized diluents. In conclusion, the FH bull had the best post thawing motility and viability compared to Simmental and Brahman bull. Based on the Indonesian National Standard all sample could be used for insemination.