Physiology Response of Juvenile Pangasius hypopthalmus on Close System Transportation With Utilization of Zeolit, Activated Charcoal and Clove Oil

Abstract

The objective of this research was to determine the effect of zeolit, activated charcoal and clove oil in maintaining water quality in the transport media and minimizing the stress level of Pangasius hypopthalmus juvenile. This research consisted of 6 treatments and 3 replications. Each treatments were; B). 20 g of zeolite + 10 g of activated charcoal; C). 20 g of zeolite + 10 g of activated charcoal + 3 ppm of clove oil; D). 20 g of zeolite + 10 g of activated charcoal + 6 ppm of clove oil; E). 20 g of zeolite + 10 g of activated charcoal + 9 ppm of clove oil; F). 20 g of zeolite + 10 g of activated charcoal + 12 ppm of clove oil, and A as a control (without zeolite, activated charcoal and clove oil). The duration of this research was 72 hours. The result showed that treatment E gave the best result among them. It was indicated by 5,348 ± 0,02 mg/L TAN (Total Ammonia Nitrogen) concentration, 50,42 mg/L CO2, 14,1 ng/ml of cortisol concentration, 1,56 (106×sel/mm3) erythrocyte, 6,52 (104×sel/mm3) leukocyte, 5,5±0,3 gr/100ml hemoglobin, 36,51±0,15 % hematocrit, 83,11% of survival rate. That treatment also showed the fewest of the gill histopathological changes and also showed the best spesific growth rate ( 1,035% of body weight/day).