Deteksi Integritas Genomik Pisang Hasil Lradiasi In Vitro Berdasarkan Penanda Mikrosatelit

Abstract

Tbe research aims to detect genomic integrity of in vitro irradiated banana using microsatellite marker. These studies were done on banana cv. Pisang Mas irradiated by 15 Gy of gamma ray. The DNA was isolated from each accesion following Dixie. Amplification of DNA products were done by Perkin Elmer Gene Amp PCR 2400 using ten primers, and then oleetroforesis in agarose I %. Finally a vertical polyacrylamide gel electroforesis was run and the products were visualized by silver staining. The result shown that among the primers tested, eight primers produced clear, discrete, and reproducible bands. Number of DNA band exhibited ranging from one to two, following the ploidy level of pi sang Mas wbich is a diploid banana cultivar (AA). One band suggest bomozygote allele while two bands showed heterozygote allele. Out of eight primers, six primers produced different allele among irradiated, in vitro, and in vivo coDtrol plant. Meanwhile, for the other two primers the allele were monomorpb for all tbe accessions examined. Genomic modification was observed at all irradiated plants. The modification can bappened at zygosity of certain allele that may change from heterozygote to homozygote or vice versa. While modification in allele size that underlying genomic instability could be caused by several genetic events sucb as deletion, insertion, and amplification of nucleotides.