Diversity of SCAR Markers of Pyricularia grisea Isolated from Digitaria ciliaris Following Cross Infection to Rice

Abstract

Cross infection of Pyricularia grisea from grass to rice and vice versa has been reported, but genetic changes are not known yet. This research aimed at estimating the possibility of the genotype alteration in P. grisea dc4 isolated from Digilaria ciliaris, following cross infection to either rice cv. Kencana bali, Cisokan, and IR64 or Panicum repens. Cynodon daetyJon, Digitaria sp., and Otloch/oa nodosa. The genotypes were analyzed by employing three SCAR markers, CutL PWL2; and Erg2. The results indicated that the dc4 was only able to infect Kencana bali, Cisokan, and P repens. The dc4 had only two oul of three SCAR markers, Cut 1 and Erg2. Host shift was followed by genotype alteration in two loci of SCAR. Iso lates derived from les ions on Kencana bali (dc4-kb) and Cisokan (dc4-c) or the dc4 infection, both lost their CUll and gained PWL2. On the contrary, there was no genotype alteration from dc4 to iso late derived from P. repens of dc4 infection (dc4-pr). Neither the iso late dc4-kb th at was cross-inoculated to Cisokan nor the dc4-c that was cross-inoculated to Kencana bali showed SCAR marker change. In comparison, race t 73 isolate and those derived from Kencana bali and Cisokan did not show genotype alteration. All had two out of three SCAR markers, PWL2 and Erg2. The isola te 173 was adapted to rice. This indicated that genotype diversity ofthe dc4 might arise following host sh ift from grass to rice.