Pengeringan kemoreaksi kultur Saccharomyces cerevisiae dengan CaO serta pengaruh sorpsi kadar air terhadap stres dan kematian kultur kering

Abstract

The aim of the research was to study chemoreaction drying of Saccharomyces cerevisiae culture and to a analyze the influence of moisture sorption on pattern of viability, stress and mortality of the dried starter culture. The cell culture of S. cerevisiae was produced in glucose media with aerobic process in a lermentor at 300 C. The cell obtained was dued using chemoreaction process. Drying was conducted by coading with various thicknes of coating and various of CaO and calcium oxide ratio used in drying. Dry culture of S. cerevisiae and proactive agent of CMC and jelly werw added to the dry culture the mixtrure then was stored at in deciators at R H 11 up 97% until water aquilibrum was achieved. Later the patterns of stress from each sample of dry culture at various conditions of moisture was analyzed. The result of the research showed that the best drying method was using coat thickness of 1 3 mm and calcium oxide 10 times the weight of dried sample. The condition of dry culture at low minimal water from 5 % were mostly in dormant state, the highest viability was at the rate ol moisture of 5 % - 8 %. but a tot portion of the cells were inactive, and at trie moisture higher than 8%, dead cells were observed. Addition 2% jelly of 2% CMC did not protect cells from stress