Peranan asam askorbik dalam meningkatkan imunitas ikan jambal siam (Pangasius hypopthalmus Fowler)

Abstract

An experiment to determine Thai catfish's (Pangasius hypophthalmus Fowler) immunity was carried out using different levels of ascorbic acid (0. 1.000. 2.000 and 3.000 mg/kg feed). Fish of 15-20 g in weight were kept in aquaria tor 6 weeks with density of 15 fish/aquaria. Peed was given at 5-10 % of total biomass with frequency of three times a day. The blood sampling was taken every week and the challenge test with Aeromonas hydrophila (106cells/mrtr) injection intramuscular was done on the 30"" day. The result of this experiment shown that feed with ascorbic acid of 2.000 mg/kg was elevated the cellular responses such as: leucocyte total (34.850 cels/mm'). differential of leucocyte (lymphocyte: 72,2%, monocyte: 8,0%, neutrophyl: 7,8%), phagocytic index (13%) and humoral response (tttrc antibody: 0.829 serum aglutination unit), which at the same time proves high level of survival rate against the artificial injection using A. hydrophila.