Biodegradasi limbah minyak berat (heavy oil waste / how) dengan teknik bioslurry menggunakan Salipiger sp. my7 dan Bacillus altitudinis my12

Abstract

Bioremediation is the application of biological treatment to clean up of hazardous chemicals, included petroleum hydrocarbon such as heavy oil waste by using microorganisms. The final design must provide the controls to manipulate the environment for enhancing biodegradation of the target compounds. One of bioremediation techniques is bioslurry which has some advantages to degrade heavy oil waste. The experiment was conducted at laboratory scale and then continued to scale up phase using bacteria Salipiger sp. MY 7 and Bacillus altitudinis MY 12. Process of biodegradation was observed in 14 days for lab scale and 28 days for scale up. In lab scale, the best combination for bioslurry was using 15% TPH and 10% solid which has 80,16% of TPH degradation. The availability of microorganisms in bioslurry bioreactor was 4,1x107 -1,6x109 CFU/ml . Acidity (pH) of the slurry was in range of 6 – 7 which is normal condition for microorganisms to grow well, and with temperature in range of 31 – 34oC. The decreasing in TPH during the scale up phase (28 days) was from 18,82% to 11,93%. Biodegradation rate can achieved at 44,57 mg/L TPH/day at first week, and 37,57 mg/L/day at second week. Acidity (pH) was 7-9 and temperature was 28oC-30o C which is normal condition for microorganisms for doing biodegradation of heavy oil waste.