Fraksionasi golongan flavonoid dari daun kepel (Stelechocarpus burahol) yang berpotensi sebagai antibakteri

Abstract

The objectives of this research was to separate flavonoids of kepel leavesfrom methanol extract as antibacterial agent. The bacteria used was Staphylococcus epidermidis that corresponds to body odor. The methanol extracts was fractionated in methanol:water (7:3), n-hexane and chloroform consecutively. Methanol:water (7:3) extract with the highest flavonoids content, fractionated by silica gel column chromatography (isocratic elution, with butanol:methanol:acetic acid 1:8:1 as eluent) to produce 7 fractions. All fractions were tested for antibacterial activity with a microplate method. Fraction V was the most active fraction with minimum inhibitory concentration (MIC) 0.06 mg/mL and minimum bactericidal concentration (MBC) of 0.50 mg/mL. Fraction V was further separated by preparative thin layer chromatography and gave three fractions. Fraction V3 was the most active fraction with MIC 1.00 mg/mL and MBC of 2.00 mg/mL. Identification of fraction V3 based on assessments on ultraviolet-visible and infrared spectrum belongs to flavones group.