Efek probiotik indigenus pada profil imunohistokimia antioksidan superoksida dismutase (SOD) di hati tikus yang dipapar enteropathogenic Escherichia coli (EPEC)

Abstract

The study was conducted to analyze influence of indigeneous probiotic Lactobacillus plantarum and Lactobacillus fermentum on immunohistochemical profile of antioxidant superoxide dismutase (SOD) in liver of enteropathogenic Escherichia coli (EPEC) treated rats. A total of 90 male rats (Sprague Dawley) were divided into six groups; (A) negative control, (B) Lactobacillus plantarum treated group, (C) Lactobacillus fermentum treated group, (D) Lactobacillus plantarum and EPEC treated group, (E) Lactobacillus fermentum and EPEC treated group, and (F) EPEC treated group (positive control). The content of antioxidant copper, zinc-superoxide dismutase (Cu,Zn-SOD) was analyzed with immunohistochemical techniques using monoclonal antibody of Cu,Zn-SOD. The result showed that L. fermentum increased the content of Cu,Zn-SOD in rat liver on 2nd and 3rd weeks at both conditions; with and without EPEC treatment. L. plantarum treatment for 1 week until 3 weeks, could maintain the content of Cu,Zn-SOD in rat liver tissue as well as negative control. While, in L. plantarum and EPEC treated group, the content of Cu,Zn-SOD decreased after one week of EPEC treatment. L. fermentum showed better effect in terms increase the content of Cu,Zn-SOD in rat liver tissue.