Kandungan ergosterol bebas tanah pada beberapa tipe penggunaan lahan di kebun percobaan Cikabayan, Dramaga

Abstract

Soil micro-organisms play a major role for the innumerable processes that occur in soil. Bacteria and fungi are two main groups of microflora us a key factor in the soil ecosystem function They influence many nutrient cycling by different ways. For better understanding about the function of micro-organisms in soil nutrient cycling, measurement of bacterial and fungal contribution to microbial biomass is needed. In this experiment, the measurement of fungal biomass using fungal biomarker ergosterol with High Pressure Liquid Chromatography (HPLC) instrument is conducted. Ergosterol is a sterol particularly useful index for fungal growth because it is endogenous only to fungi, lichens and certain micro algae, it is not produced in significant quantities by green plants. Ergosterol has a characteristic of UV light absorption it is very-different from other sterols because it has a conjugated pair of double bonds at carbon 5-6 and 7-8 has strongly UV light absorption between 300 and 240 nm with a characteristic pattern and hat maximum absorption at 282 nm. This experiment is conducted at the Soil Biology Laboratory and Mineralogy , Genesis, and. Classification Laboratory. Soil from Cikabayan Experimental Station with eight hpes of land use and two kinds of soil depth are analyzedfor soil ergosterol content. The result of carbon biomass of fungi using biomarker as ergosterol method is compared with carbon biomass of microorganism (CMc) from sonification method using correlation equations. It is ulso compared wirh carbon biomass of fungi from plate culture method using correlation equations. The result of correlation analysis between biomarker ergosterol method and sonification method is 0.690, biomarker ergosterol method and plate culture method is 0 448 It was found that Imperata grass (0 - 10 cm) has the highest ergosterol concentration. 2 03 µg g-1 of soil, and oil palm (10-20 cm) has the lowest ergosterol concentration, 0.14µgg-1 of soil.