Karakter Vegetatif Dan Reproduktif Tanaman Mutan Padi Sensitif Aluminium

Abstract

Aluminum (Al) tolerant mechanism in rice has not been fully understood. Aluminum tolerant mechanism can be studied using Al-sensitive plant originated from Al-tolerant plant. The research objective was to study the vegetative and reproductive characters of Al-sensitive rice mutant derived from rice cv Hawara Bunar (HB) that originally tolerant to Al. Seedlings of rice mutant generation M2 were screened using hydroponic technique based on Root Re-growth (RRG). Five days old seedlings were grown on minimum culture solution containing 15 ppm of Al for 72 hours and then transferred to non Al-treated culture solution for 48 hours. The value of RRG was determined based on the length difference between root seminal length after recovery and after Al stress. The selected seedlings were grown in green house according to low land rice cultivation technique. The result showed that the seminal root of Al sensitive rice mutants was not able to regrow during period of recovery. The screening result obtained 13 numbers of Al-sensitive mutants in M2 generation. Mutant number 20-11 had the lowest of RRG average value and the highest percentage of mutants. Vegetative and reproductive characters of rice mutant generation M2 were varied significantly. Mutant plants similar to wild type were numbers 20-11-5, 16-20-14, and 20-11-6. The mutant numbers 20-11-5 and 20-11-6 were potential to be developed, because they were the most similar to the wild type, except the tolerance to Al. Al-sensitive mutant plants is not fully stable until the third generation.