Efektivitas berbagai dosis gliserol di dalam pengencer trls dan waktu ekuilibrasi terhadap kualitas semen baku kambing peranakan etawah

Abstract

The objectives of this research were to determine the optimum dose of glycerol in Tris extender and equilibration time in maintaining frozen semen quality of crossbred Etawah bucks. This research consist of two sections: (i) evaluation of various glycerol doses (5, 6 and 7 percent) and (ii) evaluation of various equilibration time (1, 2, 3 and 4 hours), both in tris extender. Results of the first experiment indicated that the mean percentage of post thawing motility, live, intact plasma membrane and intact acrosomal cap of spermatozoa in 6% glycerol (52.60%, 65.03%, 45.63% and 47.54% respectively)were significantly higher (P< 0.05) than in 5% (44.31%, 52.00%, 37.60% and 37.00%) and 7% glycerol (45.28%, 52.10%, 37.97% and 37.14%).