Karakteristik Ketahanan Bakteri Asam Laktat Indigenous Dadiah Sebagai Kandidat Probiotik Pada Kondisi Saluran Pencernaan in vitro

Abstract

Probiotic bacteria defined as living microorganisms which will confer health benefit to the host when administered in adequate amounts. The aims of this research were to study the potential of Lactobacillus plantarum D-01, Lactococcus lactis D-01, Bifidobacterium longum Y-01, and Lactobacillus acidophilius Y-01 as probiotic bacteria through its ability to survive in gastrointestinal conditions (acid conditions of stomach and the presence of bile salts in the small intestine), its resistance to antibiotics, and its antimicrobial properties against pathogen bacterias. This study initiated with assays of four tested Lactic Acid Bacterias for its ability to grow and survive in acid conditions and the presence of bile salts or antibiotics, also its antagonistic activities against indicator strains of pathogen bacterias (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Salmonella Thypimurium ATCC 14028). Result showed the ability of L. plantarum D-01, L. lactis D-01, B. longum Y-01 and L. acidophilus Y-01 to grow in acid conditions and tolerance at the presence of bile salts and antibiotics (t-test). Research found that difference species LABs tested influenced diameter of the inhibition zone against pathogen indicator bacterias (P<0.01). L. acidophilus Y-01 had the largest inhibition zone against S. Thypimurium ATCC 14028 and E. coli ATCC 25922, while B. longum Y-01 was able to produce the largest inhibition zone against S. aureus ATCC 25923 (P<0,05). Based on the characterization of four species LAB, it could be concluded that L. plantarum D-01, L. lactis D-01, B. longum Y-01, and L. acidophilius Y-01 can be used as probiotic.