Identifikasi kandungan hemolimf ulat sutera (Bombyx mori) sebagai suplemen fetal bovine serum dalam kultur sel serangga

Abstract

The use of fetal bovine serum (FBS) as nutritional supplement in cell culture is very important for cell growth. High dependence and bioethical issues on the use of FBS needs special attention. Therefore, continuous effort to search for its replacement or supplement is still needed. Hemolymph silkworm has been reported that it contains many nutrients needed for the process of metamorphosis. This can be used as an alternative nutritional supplement for cell culture, so it can reduce the use of FBS. Hemolymph macrocomponent analysis, separation of protein fractions, and tested as medium supplement for Spodoptera fugiperda (Sf9) cell culture were performed. Hemolymph has 89.76% moisture content; 2.52 mg/mL carbohydrate; 2,35% fat; and 55.61 mg/mL protein. Further protein analysis revealed that, it consists of 15 fractions containing molecular weight from 22 to 152 kDa. The use of hemolymph on Sf9 cell culture in this study showed that it did not support cell growth. Treatment without adaption and hemolymph oxidation during cell culture could be the cause of poor growth of Sf9 cells.