Penggandaan skala produksi bioinsektisida bacillus thuringiensis var. israelensis untuk membasmi jentik nyamuk aedes aegypti
dc.contributor.author | Rahayuningsih, Mulyorini | |
dc.contributor.author | Syamsu, Khaswar | |
dc.contributor.author | Darwis, Abdul Aziz | |
dc.contributor.author | Purnawati, Rini | |
dc.date.accessioned | 2011-06-20T07:29:27Z | |
dc.date.available | 2011-06-20T07:29:27Z | |
dc.date.issued | 2007 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/46454 | |
dc.description.abstract | The objective of this research is to study the scaling up of bionsecticide production from Bacillus thuringiensis var. israelensis using onggok (a cassava by-product) as a carbon source. The insecticide produced was used to eradicate Aedes aegypti larvae. The product was a crystal protein produced during bacterial sporulation. Scaling up from laboratory to pilot plant scale was done using two methods, i.e. constant agitation power per unit volume (Pg/V) and constant oxygen transfer coefficient (kLa). The results showed that yield of product per substrate (Yp/s) of Pg/V based product with the value of 3.52 + 0.02 spora per gram substrates was higher than Yp/s of kLa based product with the value of 2.96 spora per gram substrate. Logarithmic value of viable spore count (log of VSC) was also higher, i.e. 7.23 ± 0.30 for Pg/V based product as compared to 7.17 + 0.20 for kLa based product. Substrate efficiency was also higher in Pg/V based (92.47%) than kLa based (64.87%). LC50 of Pg/V based product was lower (0.49 µg/mL) meaning that it was more toxic than kLa based product (0.62 µg/mL). Amino acid content of Pg/V based product was also higher than kLa based product. Constant Pg/V method was suggested as a based on the scaling up of bioinsecticide production of B. thuringiensis israelensis on industrial scale. | en |
dc.publisher | IPB (Bogor Agricultural University) | |
dc.title | Penggandaan skala produksi bioinsektisida bacillus thuringiensis var. israelensis untuk membasmi jentik nyamuk aedes aegypti | en |