Deaktivasi tanin kaliandra (Calliandra calothyrsus) menggunakan mineral Fe dan Cu (In vitro)

Abstract

The experiments were conducted to investigate the potency of minerals Fe and Cu in deactivating the negative effect of tannins extracted from calliandra leave. There were major and minor treatments. The major treatment used factorial randomized complete block design (2x2~3)F. irst factor was tannin content; second factor was type of minerals (Fe or Cu); third factor was level of minerals (Fe: 0, 5.6, 11.2 pprn and CLI: 0, 50.8, 101.8 ppm). Minor treatment used randomized complete block design to investigate the effect of sulphate on mineral Fe and Cu supplementation. Casein and cellulose were used as substrates in both experiments. Data were analyzed statistically wit11 ANOVA and contrast orthogonal. Parameters measured were VFA and ammonia concentration, dry matter digestibility of casein and cellulose, free mineral of Fe and Cu. Results show that Fe supplementation at level % (5.6 ppm) did not significantly increase casein digestibility in the presence of tannins. However, Fe supplementation sinergically with tannins decreased cellulose digestibility (P<0.01). Fe supplementation on casein substrate did not increase NH3 concentration in the presence of tannins, but increased total VFA concentration. Cu supplementation at level % (50.8 ppm) and 1 (101.6 ppm) was toxic to rumen microbes, so there was no cellulose degradation, NI33 production, and very low total VFA concentration. Cu supplementation did not affect casein dry matter digestibility. After 48 hours of incubation, there was a complex of tannins and minerals as indicated by reduction of free minerals in the solution (P<0.05). It is concluded that supplementation of Fe at level 5.6 pprn is recommended because it significantly reduced the negative effect of tannins by increasing total VFA and dry matter digestibility on casein substrate. Cu supplementation at level 50.8 pprn or 101.6 ppin was toxic to rumen microbe