Rekayasa Bioproses Produksi Bioetanol dari Hidrolisat Pati Sagu (Metroxylon sp.) Menggunakan Saccharomyces Cerevisiae Var.ellipsoides Pada Kultivasi Nir-Sinambung dan Semi Sinambung:

Abstract

Starch of sago is one of the sources of ethanol which is able to be produced by enzymatic hydrolysis and followed by cultivation process. It has high concentration of carbohydrate (80 %), so it can produce glucose highly with enzymatic hydrolysis process. The total amount of glucose has correlation with amount of ethanol, in cultivation process. The first step of research was to prepare substrate from sago starch which was hydrolysed enzymatically using α- amylase and amyloglucosidase (AMG) enzymes. Preliminary research was conducted to determine optimum concentration in which S.cerevisiae var.ellipsoides can live and produce the highest ethanol content. The concentrations being tested were 18, 24, 30 and 36 % (g/L) of total sugar. The main research consists of two systems: batch and fed-batch. Result shows that 24 % concentration (g/L) was find to be the optimum consentration for S.cerevisiae var.ellipsoides with ethanol production reaches up to 10,2 ± 0,000 % (w/v). One of the main research is using batch cultivation. The best result of highest ethanol concent was reached (10,77 ± 0,000 % w/v) when aeration was stopped after biomass condition has reached the maximum condition. The best result on fed-batch cultivation with the highest ethanol concentration (12,05 ± 0,00 % w/v) also was gained at the same treatment with feed concentration of glucose 16 % g/L. Based on calculation of cultivation kinetic parameters, it is shown that in batch cultivation the highest Yp/s, Yp/x (0,15; 0,47; 4,48) was gained on the third treatment that is aeration stopped at 30th hour. On fed-batch cultivation, the highest Yp/s and Yp/x value (0,51 and 5,05) is shown on treatment of which aeration is stopped after 30th hour and feed concentration of 16 % g/L.